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M9901
Sigma
Mutanolysin from Streptomyces globisporus ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
| CAS Number: | 55466-22-3 |
| MDL number: | MFCD00131635 |
Description
| Application | Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. |
| Other Notes | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer |
| Unit Definition | One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate. |
| Physical form | Lyophilized powder containing Ficoll® and sodium succinate buffer salts |
| Biochem/physiol Actions | Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus. |
| Legal Information | Fiberfrax is a registered trademark of Unifrax Corp. |
Properties
| form | lyophilized powder |
| mol wt | mol wt 23 kDa |
| EQP level | Premium |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
Related Products
| Substrate | M3440, Mutanolysin Assay Substrate |
References
| reference | Assaf and Dick, Spheroplast formation and plasmid isolation from Rhodococcus spp. Biotechniques 15(6), 1010, (1993) |
| Yokogawa, K., et al. Agric. Biol. Chem. 39, 1533-1543, (1975) | |
| Kawata, S., et al. Agric. Biol. Chem. 47, 1501-1508, (1983) | |
| Mikkonen, M., et al., Defective site-specific integration elements are present in the genome of virulent bacteriophage LL-H of Lactobacillus delbrueckii. Appl. Environ. Microbiol. 62, 1847, (1996) | |
| Siegal, J.L., Mutanolysin-induced spheroplasts of Streptococcus mutants are true protoplasts. Infect. Immun. 31, 808, (1981) |
